| Dual Enhancement of Thermostability and Activity of Xylanase through Computer-Aided Rational Design |
| 論文作者 |
Lu, MZ; Li, ZH; Zhuang, H; Yang, SH; Zhao, XC; Feng, RR; Shen, HY; Kovalevsky, A; Zhang, SK; Xie, ZP; Li, X; Shen, QR; Wan, Q |
| 期刊/會(huì)議名稱 |
ACS SUSTAINABLE CHEMISTRY & ENGINEERING |
| 論文年度 |
2024 |
| 論文類別 |
|
| 摘要 |
In the realm of enzyme engineering, the dual enhancement of thermostability and activity remains a challenge. Herein, we employed a computer-aided approach integrating folding free energy calculations and evolutionary analysis to engineer Paecilomyces thermophila xylanase into a hyperthermophilic enzyme for application in the paper and pulp industry. Through the computational rational design, XynM9 with superior thermostability and enhanced activity was designed. Its optimal reaction temperature increases by 10 degrees C to 85 degrees C, its T-m increases by 10 degrees C to 93 degrees C, and its half-life increases 11-fold to 5.8 h. Additionally, its catalytic efficiency improves by 57% to 3926 s(-1) mM(-1). Molecular dynamics simulations revealed that XynM9 is stabilized by more hydrogen bonds and salt bridges than wild-type xylanase. The mutant's narrower catalytic cleft enhances the substrate-binding affinity, thus improving the catalytic efficiency. In harsh conditions at 80 degrees C and pH 10, using XynM9 significantly reduced both hemicellulose and lignin, which makes it a good candidate for use in the paper and pulp process. Our study presents an accurate and efficient strategy for the dual enhancement of enzyme properties, guiding further improvement of computational tools for protein stabilization. |
| 期 |
41 |
| 卷 |
12 |
| 影響因子 |
7.1 |
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